Complete Amino Acid Sequence of Chitinase - A from Leaves ef
نویسندگان
چکیده
peptides were put in order. Of seyen cysteine residues, six were linked by disulfide bonds (between Cys25 alld Cys74, Cys89 and Cys98, and Cys195 and Cys208); Cys176 was free. The enzyme consisted of 208 amino acid residues and had a molecular weight of 22,391. It consisted of only one polypeptide chain withellt a chitin-binding domai". The length of the chain was almost the same as that of the catalytic demains of class IL chitinases. These findings suggested that this enzyme is a new kind ef class IIL chitinase, altheugh its seqllence resembles that of catalytic demains of class IL chitinases more than that ef the class IIL chitinases reported so far. Discussion on the inyo}yement of specMc tryptophan residue in the actiye site of PLC-A is also giyen based on the sequence similarity with rye seed chitinase-c.
منابع مشابه
Complete Amino Acid Sequence of Chitinase - A from Leaves ef Pokeweed
peptides were put in order. Of seyen cysteine residues, six were linked by disulfide bonds (between Cys25 alld Cys74, Cys89 and Cys98, and Cys195 and Cys208); Cys176 was free. The enzyme consisted of 208 amino acid residues and had a molecular weight of 22,391. It consisted of only one polypeptide chain withellt a chitin-binding domai". The length of the chain was almost the same as that of the...
متن کاملAntifungal Activity of Heterologous Expressed Chitinase 42 (Chit42) from Trichoderma atroviride PTCC5220
The cDNA from the mycoparasitic fungus Trichoderma atroviride PTCC5220 encoding a 42 kDa chitinase (Chit42) was isolated. The nucleotide sequence of the cDNA fragment as having a 1263 bp open reading frame that encodes a 421 amino acid polypeptide, and a high homology was found withother reported Chit42 belonging to the Trichoderma sp. The 22 amino acid N-terminal sequence is a putative s...
متن کاملThe Complete Amino Acid Sequence of Chitinase - a from the Seeds of Rye ( Slecale cereat
The complete amine acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleayed with cyanogen bromide and the resulting three fragments, CBI, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CBI were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin ef reduced SLcarboxymethylated ...
متن کاملComplete amino acid sequence of chitinase-A from leaves of pokeweed (Phytolacca americana).
The complete amino acid sequence of pokeweed leaf chitinase-A was determined. First all 11 tryptic peptides from the reduced and S-carboxymethylated form of the enzyme were sequenced. Then the same form of the enzyme was cleaved with cyanogen bromide, giving three fragments. The fragments were digested with chymotrypsin or Staphylococcus aureus V8 protease. Last, the 11 tryptic peptides were pu...
متن کاملIsolation and Characterization of the Genes Encoding Basic and Acidic Chitinase in Arabidopsis thaliana.
Plants synthesize a number of antimicrobial proteins in response to pathogen invasion and environmental stresses. These proteins include two classes of chitinases that have either basic or acidic isoelectric points and that are capable of degrading fungal cell wall chitin. We have cloned and determined the nucleotide sequence of the genes encoding the acidic and basic chitinases from Arabidopsi...
متن کامل